Coding

Part:BBa_M36762

Designed by: Andrew Lee, Clinton Olivas, Daniel Hu   Group: Stanford BIOE44 - S11   (2014-10-23)

Chicken Lysozyme C (LYZ) conjugated with Albumin Signal Peptide

Our part can be transformed into yeast for the production of mammalian lysozyme. Chicken lysozyme C (LYZ) has been shown to have high catalytic activity against gram-positive bacteria, hydrolyzing the peptidoglycan in the bacterial cell wall. The albumin signal peptide is optimized for lytic enzyme secretion in yeast expression hosts. By granting ethanologens, like yeast, the ability to secrete lysozyme, this part can be used to simultaneously decontaminate broths while carrying out fermentation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 434
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None